Mops Biological Good′ S Buffer CAS# 1132-61-2

Basic Info

Model NO.	DS-CP24
CAS No.	1132-61-2
Formula	C7h15no4s
EINECS	214-478-5
Classification	Biochemical Reagents
Grade	AR
Specific Usage	For Biological Purpose, For Tissue Medium Purpose, For Microbiological, For Microscopic Purpose
Content	Standard
Usage	Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents
Source	Dry Powder
Habit Appellation	Fine Chemicals
Application	Industry, Scientific Research, Health, Environmental Protection
Property	Biochemical Reagent
English Name	3-Morpholinopropane Sulfonic Acid
Abbreviation	Mops
Molecular Weight	209.26
Molecular Formula	C7h15no4s
Function	pH Adjustment
Scope	Good′s Buffers
Transport Package	25kgs Per Drum
Specification	Purity-99.5%
Trademark	DESHENG
Origin	Ezhou, China
HS Code	2934999099
Production Capacity	50tons/Month

Product Description

3-morpholinopropane sulfonic acid MOPS Biological Buffer CAS# 1132-61-2
(English name)3-Morpholinopropane sulfonic acid
(Chinese name)3-Morpholinopropane sulfonic acid
(Abbreviation)MOPS
(CAS#)1132-61-2
(Molecular weight)209.26
(Molecular formula)C7H15NO4S
(Storage condition)room temperature, away from light and moisture
(Molecular structure)(Application)Mops is a kind of biological buffer, which is often used to prepare RNA electrophoresis buffer.
(Product advantage)The purity is ≥99%, with good water solubility and stable process, which can ensure that the product appearance is pure white crystal powder.MOPS (3-(-morpholino)propanesulfonic acid) is a buffer introduced in the 1960s, one of the twenty Good's buffers. It is a structural analog to MES,^[1] and like MES, its structure contains a morpholine ring. HEPES is a similar pH buffering compound that contains a piperazine ring. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.

Applications

MOPS is frequently used as a buffering agent in biology and biochemistry. It has been tested and recommended for polyacrylamide gel electrophoresis.^[2] Usage above 20 mM in mammalian cell culture work is not recommended.^[3] MOPS buffer solutions become discolored (yellow) over time, but reportedly slight discoloration does not significantly affect the buffering characteristics.^[4]

MOPS Buffer (10X) (0.2 M, pH 7) Preparation and Recipe

MOPS buffer is often used in polyacrylamide gel electrophoresis. To prepare L of MOPS Buffer (10X) (0.2 M, pH 7):Change the value in the textbox above to scale the recipe volume Table 1. Required components

Component	Amount	Concentration
MOPS free acid (mw: 209.26 g/mol)	41.86 g	0.2 M
Sodium Acetate (mw: 82.03 g/mol)	4.1 g	0.05 M
Disodium EDTA (mw: 372.24 g/mol)	3.72 g	0.01 M

Prepare 800 mL of dH2O in a suitable container.
Add 41.86 g of MOPS free acid to the solution.
Add 4.1 g of Sodium Acetate to the solution.
Add 3.72 g of Disodium EDTA to the solution.
Adjust solution to desired pH using NaOH (typical pH = 7)
Add dH2O until the volume is 1 L.

MOPS is a zwitterionic biological buffer often used in biological and biochemical research. After reviewing several academic papers in which MOPS buffer was studied, we would like to share with you some recommendations and concerns you must have when using this buffer. We hope you find these tips useful!

MOPS basic information:

CAS Number: 1132-61-2
Molecular Weight: 209.3
Formula: C₇H₁₅NO₄S
Useful ph range: 6.5 - 7.9
pKa (25°C): 7.0 - 7.4
Price / Specifications: Click here

What is MOPS recommended for?

For denaturing gel electrophoresis of RNA¹
For protein purification in chromatography
To measure absorption during UV/VIS spectrophotometry and to study redox properties using cyclic voltammetry²
In nitrogenase electron transfer mechanism³
In the separation of nucleic acids and proteins by electrophoresis⁴
In the control of culture media pH⁵, including cell culture media for yeast, bacteria and mammalian cells.
It has been used as a buffer component of charcoal yeast extract medium⁶
It interacts with peptide backbone of bovine serum albumin leading to a net stabilization of the protein⁷

Which concerns should you have before choosing MOPS for your research?

MOPS concentration should be not higher than 20mM when used for mammalian cell culture
Although most studies found little complexation between Mops and metals, some studies found that interferences may occur due to metal complex formation⁸
It can modify lipid interactions⁹
MOPS can influence the thickness and barrier properties of rat endothelial surface layers¹⁰
It interacts and forms complexes with DNA¹¹
It can slightly affect mRNA expression of in vitro produced bovine embryos¹²
Mops interferes with Lowry protein determination
It can be oxidized by H₂O₂, but since buffer oxidation is slow, no significant impact on biological systems is expected to happen¹³
MOPS is partly degraded on autoclaving in the presence of glucose

Useful tips about MOPS:

It does not interfere with Bradford or bicinchoninic acid assays14

Desheng MOPS Buffer

Desheng Biochem is among the largest producers of MOPS in the world. Our products are shipped daily to top research centers and biotech companies in Europe, America and Asia.

What we offer:

Product straight from our manufacturing sites
Worldwide shipping to your door
Assistance with shipping
Small and bulk packages (from grams to tons)
International quality standards
After-sales service with English speaking staff

Check the specs and price of Desheng MOPS

Products

Mops Biological Good′ S Buffer CAS# 1132-61-2

Basic Info

Product Description